Evaluation of process parameters in shake flasks for mammalian cell culture
نویسندگان
چکیده
Introduction Shake flask cultivation is nowadays a routine technique during process development for mammalian cell lines. During shaken culture, changes in agitation velocity, shaking diameter or shake flask size affect the hydrodynamics in the shake flask. This might be reflected in the growth of the cultured cells. Process parameters such as power input, mixing time, fluid velocity etc. have been determined and described mathematically for shake flasks used for microbial cultivation, but only to some extend for mammalian cell culture. Especially the relationship between these parameters and growth characteristics of mammalian cells is still a relatively uncovered issue. In this work, process parameters like specific power input, mixing time, maximum fluid velocity and Reynolds number were determined for four different shake flasks (baffled and unbaffled) in a range of shaking velocities on a shaking machine. The specific growth rate (μmax) of the human industrial cell line AGE1.HN ® (ProBioGen AG, Berlin, Germany) was compared to the respective process parameters.
منابع مشابه
Comparison of the behavior of CHO cells during cultivation in 24-square deep well microplates and conventional shake flask systems
In biopharmaceutical production, the optimization of cell culture media and supplementation is a vital element of process development. Optimization is usually achieved through the screening of multiple media, feed and feeding strategies. However, most screening is performed in shake flasks, which makes the screening process very time consuming and inefficient. The use of small scale culture sys...
متن کاملFlow cytometry and phytochemical analysis of a sunflower cell suspension culture in a 5-L bioreactor.
A cell suspension culture of sunflower (Helianthus annuus), a producer of immunologically active polysaccharides, was cultivated in a 5-L stirred tank bioreactor, operated in batch mode. After some changes in the internal bioreactor design a stable growth of Helianthus cells was achieved and the accumulated biomass reached 15.2 g/L (only approximately 5% lower compared to the accumulated biomas...
متن کاملUse of Micro Bioreactor systems to streamline cell line evaluation and upstream process development for monoclonal antibody production
Introduction The development of monoclonal antibody (mAb) processes conventionally involves the generation of multiple cell lines in multiwell plates, cell line screening in shake flasks followed by final cell line selection and process optimisation in bioreactors. This process can typically take up to 18 months to generate a robust process suitable for early phase manufacturing and therefore a...
متن کاملEvaluation of the advanced micro-scale bioreactor (ambr™) as a highthroughput tool for cell culture process development
Introduction Bio-pharmaceutical industries face an increasing demand to accelerate process development and reduce costs. This challenge requires high throughput tools to replace the traditional combination of shake flasks and small-scale stirred tank bioreactors. A conventional and widely used process development tool is the stirred tank reactor (STR) ranging from approximately 1L to 10L in wor...
متن کاملDoE of fed-batch processes – model-based design and experimental evaluation
Background Experimental process-development and optimization is expensive and time-consuming. Real optimization by means of design of experiments involves data generation before optimization can be aimed for. This can make the way from process development to process establishment even harder, since academia or start-up research facilities might not have the possibility to generate these data. F...
متن کامل